A newly formed cross-disciplinary task force aims to codify best practices in spatial transcriptomics, establishing standards for segmentation quality control and multimodal integration benchmarks. This initiative responds to a consensus on the maturation of single-cell analyses, which are overcoming artifact risks and enhancing research reliability. As a result, researchers are poised to utilize advanced computational frameworks and shared gold standards like RNA FISH to drive innovation in cancer and disease research.
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“The results revealed that the TGF-β signaling pathway exhibited the highest activity at the tumor-stroma interface, which was enriched with cancer-associated fibroblasts (CAFs), immunosuppressive cells, and genes related to extracellular matrix (ECM) remodeling.”
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“Functional assays demonstrated that SLC20A1 enhances the proliferation, migration, and epithelial-mesenchymal transition (EMT) of HCC cells, whereas its knockout significantly suppresses these malignant phenotypes.”
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“This study represents the first comprehensive integration of spatial and single-cell transcriptomics to uncover the spatial organization of TGF-β signaling in HCC and to identify the TGF-β-SLC20A1 axis as a critical driver of tumor invasion at the tumor-stroma interface.”
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“The CTHRC1+ CAF subtype was significantly associated with T cell exclusion and upregulated expression of immune checkpoint genes.”
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“High infiltration of both cell types synergistically correlates with worse prognosis and unfavorable response to immunotherapy.”
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“The THBS2-SDC4 signaling pathway between CTHRC1+ CAFs and MMP7+ epithelial cells acts as a potential therapeutic target to disrupt protumorigenic crosstalk and improve clinical outcomes for CRC patients.”
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“Pressure overload-induced myocardial hypertrophy is associated with complex spatial and temporal remodeling of cardiac cell populations.”
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“Integrating spatial transcriptomics with single-cell RNA sequencing enables a comprehensive characterization of cardiac remodeling at high cellular and spatial resolution.”
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“Integrated spatial and single-cell analyses identified cluster 1 as a key microenvironment undergoing dynamic remodeling, driven predominantly by T cells and macrophages.”
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“The findings highlight dynamic immune-stromal interactions during pressure overload-induced remodeling and identify Lars2 as a gene associated with disease progression.”
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“Idiopathic pulmonary fibrosis (IPF) is a progressive, fatal lung disease with limited treatment options and a poor prognosis.”
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“Across integrated public bulk, single-cell, spatial, and blood multi-omics, CXCL13, IL33, TLR4, and IGF1 were identified as core IPF genes consistently linked to immune infiltration and fibrotic remodeling.”