In response to potential inaccuracies in computational segmentation of tumor cells, experts are now mandating validation through subcellular-resolution imaging or spike-in controls to mitigate transcript bleed risks. While advancements in HOC-FD and CmTSA technologies have propelled analyses of tumor microenvironments, the necessity for algorithm benchmarking with known cell boundaries has become clear to ensure reliable biological decision-making. The adoption of this cautious risk posture marks a pivotal shift in the pursuit of accurate cancer diagnostics.